Estimation of guggulsterone E and Z in the guggul-based commercial formulations using high-performance thin-layer chromatography

Quantitative Estimation of Efavirenz by High Performance Thin Layer Chromatography

A simple, accurate, and precise HPTLC method has been developed and validated for the estimation of efavirenz from bulk drug and capsule formulations. The separation was achieved on TLC plates using an appropriate solvent system. The spots so developed were densometrically scanned at 247 nm. The linearity of the method was found to be within the concentration range of 1-40 μg/ml. The validation...

Qualitative Metabolism Assessment Using High Performance Thin Layer Chromatography

Determination of active ingredient in synthetic pyrethroid formulations by high-performance thin-layer chromatography/densitometry.

A new, simple, precise, and rapid high-performance thin-layer chromatography (HPTLC) method was developed for the analysis of commercial emulsifiable concentrate (EC) formulations of cypermethrin, alpha-cypermethrin, and lambda-cyhalothrin. A known amount of reference grade synthetic pyrethroid and its EC formulation was subjected to TLC, and the amount of pesticide present in the active ingred...

Estimation of urinary aldosterone using thin-layer chromatography and fluorimetry.

AIMS To develop a fluorimetric method for the estimation of urinary aldosterone; to establish a normal range in 24 hour and overnight urine samples; and to investigate the use of overnight urines for detecting hyperaldosteronism. METHODS Essential steps include hydrolysis of the 18 conjugate to release aldosterone and its oxidation with Benedict's solution, followed by thin-layer chromatograp...

Sensitive and accurate estimation of losartan potassium formulation by high-performance thin-layer chromatography

AIM AND OBJECTIVES To develop a simple, sensitive, cost-effective and reproducible UV-spectrophotometric method and validate for the estimation of disodium edetate in topical gel formulations. MATERIALS AND METHODS Solution of disodium edetate reacts with ferric chloride to form complex in 0.1 N HCl giving λmax at 270 nm. Beer's law was obeyed in the concentration range of 5-50 μg/mL (r (2) =...